Gel Shift Binding Buffer 5X Solution

Gel Shift Binding Buffer 5X Solution

Gel Shift Binding Buffer 5X Solution is a biotechnology-grade reagent formulated to support electrophoretic mobility shift assays (EMSA), a widely used technique for studying proteinnucleic acid interactions. Supplied as a 5X concentrate in four 500 L aliquots, this buffer maintains optimal ionic strength and pH (7.5) to preserve binding specificity and complex stability during gel electrophoresis. Its clear, ready-to-use formulation ensures consistent performance across DNA, RNA, and protein-binding studies.

Researchers in molecular biology, gene regulation, and transcription factor analysis rely on EMSA buffers to detect and quantify nucleic acidprotein complexes. This buffer is designed to minimize background noise and enhance resolution of shifted bands, enabling accurate interpretation of binding events. Filtered through 0.22 m membranes and prepared with ultrapure water, it ensures high purity and reproducibility. With a shelf life of one year and storage at 20C, it integrates seamlessly into manual and automated workflows for gel-based interaction assays.

Key benefits:

• 5X concentrate optimized for EMSA workflows

• Maintains pH 7.5 for stable proteinnucleic acid interactions

• Supplied in 4 500 L aliquots for flexible use

• Filtered through 0.22 m membranes using ultrapure water

• Stored at 20C with 1-year shelf life for long-term reliability

Products are for in vitro research use only (RUO).

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